Progress has been made in the systematic discovery and evaluation of long-chain non-coding RNA-encoded polypeptides
The author:Go Top Peptide Biotech    Released in:2021年07月08日
摘要:Molecula r&Cellula rProteomics released online research papers on the research paper Deeply Mining of the full elite team of researchers from the Chinese Academy of Sciences Biophysics Research Laboratory and the elite team of academicians Chen Runsheng of the Chinese Academy of Sciences on the system of long-chain non-coding RNA (lncRNA) encoding polypeptides aU niverseof PeptidesEncodedbyLon gNoncodingRNAs.

Molecula r&Cellula rProteomics released online research papers on the research paper Deeply Mining of the full elite team of researchers from the Chinese Academy of Sciences Biophysics Research Laboratory and the elite team of academicians Chen Runsheng of the Chinese Academy of Sciences on the system of long-chain non-coding RNA (lncRNA) encoding polypeptides aU niverseof PeptidesEncodedbyLon gNoncodingRNAs.

lncRNAs are considered to be a type of non-coding RNA transcripts that exceed 200 bases in length and do not encode proteins. As everyone knows, research has shown that small open reading frames (small openreadin gframe, sm ORFs) in many lncRNA s transcripts can encode polypeptides (sm ORFen codedpolypeptides, SEPs), and the latter one can generally participate in body muscle production, mucosa The whole process of molecular biology, such as immunity, RNA removal, and tumor reproduction.

Progress has been made in the systematic discovery and evaluation of long-chain non-coding RNA-encoded polypeptides


Fully considering the huge total number of lncRNA s transcripts and sm ORF s, SEPs may mean a treasure containing protein vitality adjustment factors that have been neglected and yet to be developed and designed. To

Therefore, the large-scale discovery and identification of SEPs and systematic exploration of their functions and effects in the evolution of life can reveal the methods and expressions of genetic material transmission by non-coding RNA receptors and the research on the management and control of the Internet. Different from the perspective of protein coding and genetic genes, it is the structure and function of genes to give new breakthrough points.

The large-scale discovery and identification of SEPs still encounter challenges: the traditional relativity of lncRNA among species is relatively weak, and it has the specificity of institution and time, which promotes the institution and time expression of lncRNA encoding polypeptides to have strong dynamics; at this stage The number of SEPs discovered and identified is relatively limited, and it is difficult to encode the biological characteristics of the polypeptide in lncRNA, such as sequence, information content, traditional, physical properties (such as the reliability of RNA and polypeptide), structure type, gene location information and transcription The structure of the book itself has been structured and explored, resulting in the discovery and identification of SEPs based on bioinformatics, and the research on their functions are still challenging; the Chinese translation and control system of SEPs is not clear, and some studies have reported that the Chinese translation of SEPs is not thorough Following the AU G start and end standards, only Chinese translations with AU G as the start and end exist, indicating that SEPs are likely to have some unique Chinese translations and control systems; lncRNA encoding polypeptides are highly sensitive and high-throughput sequencing. The appraisal work ability needs to be improved.

Regarding the above limitations and challenges, Yang Fu's entire research group and Chen Runsheng's research group collaborated, and based on the search of the NONCODE database, the sm ORF in the transcript of the lncRNA of the human and the mouse, the system was explored, and each set up a system with 3.97 million and 871 Thousands of targets, humans and mice, potential SEP basic theory, database query, and systematic integration of peptide aggregation strategies based on molecular weight, membrane filtration, and solid-phase extraction, and created high-precision, high-throughput sequencing based on microbial mass spectrometry, lncRNA encoding polypeptides Discovery and identification of technical service platforms.

Research staff used this technical service platform to obtain the following results: (1) Among 8 human tumor cell lines, 3 mouse cell lines, and 8 physical and mental health institutions, 762 were found and identified with high authenticity The high-level SEP is the largest number of identifications known at this stage based on the technical SEP data of microbial mass spectrometry analysis; (2) Research shows that some SEPs are expressed in a variety of cell lines or institutions, and most SEPs are only in A certain or a certain type of cell strain and institution has been identified, with somatic cell or institution specificity spread all over; (3) Human lncRNA transcripts encoding SEP are key from genetic inter-lncRNA (48.6%), 18.6% And 17.7% each come from exon lncRNA and antisense lncRNA, a small part comes from sense non-exon lncRNA (15.1%); (4) start codon statistical analysis data shows that only 28% of human origin The start codon of lncRNA encoding polypeptide is AUG, while 67% of human SPEs have non-AUG start codons. To

The above scientific research results will provide a certain basis of data information for the study of SEP's translation and control system, and the data and basic theories given for the system discovery and function identification of genes in non-coding RNA and genetic genes are applicable.


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