Peptide detection


N-terminal (Lys side chain) and C-terminal modifications

Free amino group on the peptide chain is a common peptide modification site, Baiyicin provides a variety of modification methods, so that the design of the peptide chain diversification:

Acetylation (Acetylation)

Formylation (Formylation)

Biotin labeling (Biotin)

Fatty acid modification (Palm, Myr,Lauryl)

TFA, BZO, ABZ, CLAC, SUC

Hynic

Lipoic

DOTA

NOTA

DOXORUBICIN


Phosphorylation marker

Phosphoropeptides play an important role in the process of life, and there are three common phosphorylation sites: p-Tyr, p-Ser, and p-Thr.


D type amino acid modification

Common amino acids: D-Ala, D-Arg, D-Asn, D-Asp, D-Cys, D-Gln, D-Glu, D-His, D-Ile, D-Lys, D-Leu, D-Met, D-Phe, D-Pro, D-Ser, D-Thr, D-Trp, D-Tyr, D-Ser

Very common amino acids: D-AIB, D-ABU, D-BAL, D-CHA, D-CIT, D-HYP, D-NLE, D-NAL, D-NVA, D-ORN, D-TIC, D-PEN

 

Methylation modification

In epigenetics, the methylation of Arg and Lys makes the protein of tissue epigenetics repress or activates gene expression.

Methylation modifications on other amino acids can change the biological activity or stability of peptides. We provide a variety of methylation modifications to meet your drug design and development needs:


N-methylation modification:

NME-ALA, NME-Asn, NME-Asp, NME-Arg, NME-Cys, NME-Glu, NME-Gln, NME-Leu, NME-Lys, NME-Ser, NME-Thr, NME-Trp, NME-Val, NME-Nle

Other sites of methylation modification:

Lys(ME), Lys(ME)2, Lys(ME)3, Arg(ME), Arg(ME)2S, Arg(ME) 2As, Cys(ME), Asp(OME), Glu(OME), Tyr(ME), Trp(ME)


 


Polyethylene glycol modification

Polyethylene glycol (PEG) is a neutral, non-toxic polymer that has unique physical and chemical properties and good biocompatibility. It is also one of the few synthetic polymers approved by FDA that can be used in vivo for medicinal purposes.

Polyethylene glycol, or PEG, is highly hydrophilic, has a large hydrodynamic volume in aqueous solutions, and has no immunogenicity.

When lotus root to the drug molecule or surface, its good properties can be given after the modification of drug molecules, change their distribution of biological behaviour and solubility in the aqueous solution on its modification drugs produce space around barriers, reduce drug enzymolysis, avoid quickly eliminate in kidney metabolism, and make the drugs can be detected cells of the immune system;

PEG modifications derived from peptides include:

PEG2, PEG4, PEG8, PEG12, PEG24

PEG2000, PEG5000, PEG3400

PEG20K, PEG40K


Protein conjugation of polypeptide

Small peptides/haptens must be coupled to the carrier protein for efficient antibodies to be produced.

In general, polypeptides can be conjugated to proteins under the following conditions: there is a free amino or carboxyl group;-SH on cysteine can also be conjugated to carrier proteins.

The protein conjugates provided by Baiyicin are:

KLH coupling

BSA coupling

OVA coupling


MAP complex antigen peptide

Multiple Antigen peptide (MAP) is an effective method to produce high-valent anti-peptide antibodies and peptide vaccines.A - or E - groups of lysine form the backbone of the polyantigen peptide, and the polypeptide is synthesized by the branching of the polyantigen on the outer layer.According to the number of lysine, multiple antigenic peptides with different number of side chains can be synthesized, so that antibodies with high titer and high affinity can be produced without coupling the antigen to the carrier protein.Common MAP peptides provided by Baiyexin are:

MAP4 4 branch

MAP8 8 branch

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