CodeItemDetailsDelivery standardsDelivery time(week)
AntigenPep001Peptide synthesis13-18aa,purity >85%,20mgsupply HPLC and MS2
Pep002Modified peptide synthesis2 peptide,synthesis 20mg each,purity >85%supply HPLC and MS2-3
Pep003Peptide crosslinkingCross-linked 5mg polypeptide, dialysis
1
Pro001Whole gene synthesis

According to the customer’s protein information, optimize the codons,complete gene synthesis to construct plasmid

Gene sequencing report2-3
Pro002Protein expression and purificationPerform a small amount of testing first, and then perform a large amount of expression purification.5mg,85%,SDS-PAGE detection and verification2-3
Pro003Protein concentration identificationAfter the customer provides the protein, it is evaluated, identified and concentrated.Supply SDS-PAGE report1
ImmunityImm001immune1A rabbit30-50ML serum8-10
Imm002immune22 rabbits60-100ML serum8-10
purifiedPur001Protein A affinity purification 1Purify the serum of a rabbitSupply 5-10mg antibody1
Pur002Protein A affinity purification 1Purify the serum of a rabbitSupply 2-5mg antibody1
Pur003Protein A affinity purification 2One antigen, two rabbits sera were purified separatelySupply 2-5mg antibody each1
Pur004Cross-PurifiedPurification of modified antibodies(One 10ml serum, purified twice)Supply 1-2mg Modified antibody1-2
TestingQua001Elisa-SerumTest once (1 serum)1:64000 dilution,OD>0.61
Qua002Cross-Elisa-SerumTest once (2 serum)1:64000 dilution,OD>0.6,Significant difference with non-modified peptide detection1
Qua003Elisa-PabTest once(1 purified antibody)1:64000 dilution,OD>0.6。1
Qua004Cross-Elisa-PabTest once(1 purified antibody)1:64000 dilution,OD>0.6,Significant difference with non-modified peptide detection1












Peptide   packagePep-Package001One peptide to purified polyclonal antibody 1Synthesize 1 peptide, immunize two rabbits, and select one rabbit for purification after the Elisa is qualified,Elisa qualified,2-5mg antibody,remaining serum,report14-16
Pep-Package002One peptide to purified polyclonal antibody 2Synthesize 1 peptide, immunize two rabbits, and purify the two rabbits separately after the Elisa is qualifiedElisa qualified,2-5mg antibody/rabbit,remaining serum,report14-16
Pep-Package003Two peptides to purified polyclonal antibodySynthesize 2 peptides, immunize two rabbits separately, and purify the two rabbits separately after Elisa is qualifiedElisa qualified,2-5mg antibody/rabbit,remaining serum,report14-16
Pep-Package004Modified antibodySynthesize 2 peptides and immunize two rabbits. and select one rabbit for cross-purification test after the Elisa is qualifiedElisa qualified,1-2mg modified antibody,remaining serum,report14-16
Protein  packagePro-Package001The whole gene was synthesized into a purified antibodyCustomers provide sequence information, after codon optimization, complete gene synthesis, immunization, and purification of a rabbitElisa qualified,plasmid,5-10mg antibody,remaining serum,report16-18
Pro-Package002Protein antigens to serum 1Customer provide the antigen, after identification, immunize a rabbit to the serumElisa qualified,30-50 serum,report9-10
Pro-Package003Protein antigen to purified antibody 1Customer provide the antigen. After identification, two rabbits will be immunized. After Elisa is qualified, one rabbit will be selected for purification.Elisa qualified,5-10mg antibody,remaining serum,report10-12
Pro-Package004Protein antigen to purified antibody 2Customer provide the antigen. After identification, two rabbits are immunized, and after Elisa is qualified, two rabbits are purified separatelyElisa qualified,5-10mg antibody/rabbit,remaining serum,report10-12
Pro-Package005Expression plasmid/strain to purified antibody1Customer provide expression plasmid/strain, expression purification, immunization of two rabbits, after Elisa is qualified, select one rabbit for purificationElisa qualified,5-10mg antibody,remaining serum,report12-15



Tel: +86-571-88211951 , 88211921. Fax:+86-571-88211907
Email: sales@gotopbio.com, Sales1@gotopbio.com
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