124584-08-3,Nesiritide, Polypeptide Material
Dirction：Nesiritide (CAS 124584-08-3), also referred to as human BNP-32, is a synthetic 32-amino acid polypeptide with a minimum purity of 98% determined by HPLC. Intended exclusively for laboratory research and life science experiments, not for clinical application. Customizable packaging from mg to g scale is available; store at -20℃ protected from light, ideal for receptor and signaling pathway research.
basic information:
brand: solid extension
appearance traits : white powder
storage: shading , cool drying , sealed preservation purity: ≥ 99% main use : Polypeptide Research Use
execution standard: enterprise standard
specifications: mg/g/kg, etc ( subject to customer requirements )
sales Scope: Global
peptide frequently asked questions:
1. How to calculate the theoretical net peptide content?
Most of the impurities in the polypeptide sample are counterions, and a small part contains water, solvents absorbed by the polypeptide, and some other substances. Assuming that the counterion trifluoroacetate (TFA) is the only non-polypeptide impurity in the polypeptide product delivered to you, the theoretical net polypeptide content can be estimated:
first, we divide the molecular weight of the polypeptide into two parts, one is the polypeptide and the other is the TFA counter ion of the neutralizing polypeptide. The molecular weight of TFA can be calculated by multiplying the number of ions by the molecular weight of TFA counter ion (MW = 114). If the molecular weight of the synthetic polypeptide is 1000, and the polypeptide has a free N-terminus and an Arg residue, the theoretical net polypeptide content is calculated as 1000/(1000+2 x 114 ) = 1000/1228=0.81 or 81%. Therefore, our actual polypeptide net content can usually be obtained by quantitative amino acid analysis.
2. What method is used for polypeptide synthesis?
Solid phase synthesis technology, liquid phase synthesis technology, microwave synthesis technology and recombinant extension technology. Solid extension can be based on the customer's peptide sequence characteristics, flexible selection of appropriate synthesis technology or combination.
