AF-1, KF-7-NH2, peptide synthesis

Driction：AF-1 & KF-7-NH2 synthetic peptides, HPLC purity ≥95%, C-terminal amidated for stability. Catalog peptides for neuroscience & bioactivity research, lyophilized powder, -20°C storage available.

basic information:

brand: solid extension

appearance traits : white powder

storage: shading , cool drying , sealed preservation purity: ≥ 99% main use : Polypeptide Research Use

execution standard: enterprise standard

specifications: mg/g/kg, etc ( subject to customer requirements )

sales Scope: Global

how is peptide purity calculated?

Solid Tuo is by reverse phase high performance liquid chromatography (RP-HPLC) determination of the target peptide in the peptide bond at a specific absorption wavelength of the ultraviolet absorption value of the calculation of the purity of the polypeptide, water and acetonitrile gradient elution to separation and purification. Among them, water and residual salt cannot be measured by UV detector, and other impurities exist in the detection results, including incomplete sequence peptide (short peptide with one or more amino acid residues less than the target peptide), truncated sequence (polypeptide generated by preventing the generation of incomplete sequence peptide from blocking), and incomplete deprotection peptide (polypeptide generated during synthesis or final cleavage).

What is net peptide content?

It is important to understand the difference between the net content of the polypeptide and the total weight (gross weight) of the polypeptide. In general, a polypeptide lyophilized powder sample contains not only the polypeptide, but also some other substances, such as water, solvents absorbed by the polypeptide, counter ions and salts.

The total polypeptide weight (gross weight) refers to the weight of all these mixtures.

The net peptide content is relative to non-peptidic substances, counterions and moisture, and after these are removed, the remaining is the net peptide content. The net peptide content can be determined by elemental nitrogen analysis or amino acid composition analysis and is typically 50-80% of the total peptide weight.

The net peptide content is distinct from polypeptide purity, which refers to the percentage of the polypeptide of interest to the polypeptide in the sample.