NNC0487-0111(amycretin) vendor/Peptide vendor/Peptide purification

Description

Amycretin is an experimental weight loss drug. It is a dual agonist, a single-molecule long-acting drug that acts simultaneously on GLP-1 and amylin receptors to promote weight loss. 

Specifications

Apperance: White to off-white powder

Purity(HPLC): ≥98.0%

Single Impurity: ≤2.0%

Acetate Content(HPLC): 5.0%～12.0%

Water Content (Karl Fischer): ≤10.0%

Peptide Content: ≥80.0%

Packing and Shipping: Low temperature, vacuum packing, accurate to mg as required.

FAQ：

Which end is best for my research?

By default, the peptide ends with an N-terminal free amino group and a C-terminal free carboxyl group. The peptide sequence often represents the sequence of the mother protein. In order to be closer to the mother protein, the end of the peptide often needs to be closed, that is, n-terminal acetylation and C-terminal amidation. This modification avoids the introduction of excess charge, and also makes it more able to prevent exonucliase action, so that the peptide is more stable.

How do you dissolve polypeptides?

The solubility of polypeptide depends mainly on its primary and secondary structure, the nature of modification label, solvent type and final concentration. If the peptide is insoluble in water, ultrasound can help dissolve it. For basic peptide, it is recommended to dissolve with 10% acetic acid; For acidic peptides, dissolution with 10%NH4HCO3 is recommended. Organic solvents can also be added to insoluble polypeptides. The peptide is dissolved in the least amount of organic solvent (e.g., DMSO, DMF, isopropyl alcohol, methanol, etc.). It is highly recommended that the peptide be dissolved in the organic solvent first and then slowly added to water or other buffer until the desired concentration.

What is net weight? What is peptide content?

After lyophilized peptide is generally fluffy and fluff-like, it may still contain trace amounts of water, adsorbed solvents and salts due to the characteristics of peptide itself. This does not mean that the purity of the peptide is not enough, but that the actual content of the peptide is reduced by 10% to 30%. The net weight of the peptide is the actual weight of the peptide minus the water and protonated ions. In order to ensure the concentration of peptide, the non-peptide substances need to be removed from the crude peptide.

How pure can the peptide be?

Our company can provide different purity levels for customers to choose from, from crude to > 99.9% purity. According to customer needs we can provide purity > 99.9% ultra-pure polypeptide.

What is the direction of synthesis of peptides?
Peptide synthesis is from the C-terminus to the N-terminus of the polypeptide.

Why should peptides be modified by N-terminal acetylation and C-terminal amidation?
Such modifications can give peptide sequences properties that are native to proteins.

What are the applications of peptide libraries?
Peptide libraries are an efficient tool for many studies, including GPCR ligand screening, protein-protein interaction studies, functional proteomics, nucleotide binding, screening of enzymatically acting substrates and inhibitors, antigen and epitope screening, signaling molecule search, and other important processes of drug screening.