Cell culture, also known as cell culture technology, is a technique for culturing individual cells or populations of cells in vitro. This method simulates the physiological environment in vivo, provides appropriate conditions such as nutrition, temperature and pH, and enables cells to grow, reproduce, and maintain certain structure and function in a sterile environment.
Objective
Cell culture technology has been widely used in many fields such as basic research, virus detection, drug screening, and production of vaccines and protein drugs.
Classification of
Cell culture mainly includes animal cell culture, plant cell culture and microbial cell culture. According to the growth mode, cell culture can be divided into two types: adherent growth and suspension growth.
Suspension cell culture has a large culture space and efficient cell proliferation ability, which is suitable for large-scale culture. According to the propagation characteristics of cells, they can be divided into primary cells and passage cells. Primary cells usually divide for only two to three generations, after which they begin to degenerate; The passaged cells can be continuously cultured and can be further subdivided into diploid cell lines and passaged cell lines according to their genetic characteristics. Passaged cell lines propagate rapidly and are easily preserved for a long or unlimited period.
Methods
The entire process of cell culture usually consists of several steps including preparation, culture, freezing, and resuscitation. Among them, the most critical point is that aseptic procedures must be strictly followed to ensure that the cell culture is free from contamination. In addition, factors such as nutritional conditions, temperature, pH, and gas environment are also very important.
Post time: 2025-07-18