Overview

SNAP-8 preparation methods

SNAP-8, namely acetyl octapeptide -3, is a polypeptide with N-terminal acetyl modification and C- terminal amino modification. The commercial products are generally white freeze-dried powder with high chemical stability, heat resistance, acid and alkali resistance, and soluble in water. Compared with the general structure of the peptide material, its stability is higher, is the classic class of botulinum anti wrinkle peptide in cosmetics.

Acetyl octapeptide -3 Chemical structural formula

Basic introduction

chinese name: Acetyl octapeptide -3

english name: SNAP-8

company No.: GT-C003

CAS No.: 868844-74-0

sequence: Ac-EEMQRRAD-NH2

molecular formula: C41H70N16O16S1

molecular weight: 1075.16

Acetyl Hexapeptide -3 HPLC

Physiological activity

SNAP-8 with mild anti-wrinkle, relax muscle physiological activity, its mechanism is to gently reduce neurotransmitters such as acetylcholine, catecholamine release, reduce muscle contraction tension, to reduce dynamic wrinkles, desalination shallow static wrinkles effect.

Preparation method

at present, the main methods for the synthesis of SNAP-8 and other polypeptides are chemical methods, such as solid phase synthesis and liquid phase synthesis, but there are some problems in this method:

(1) The method needs to add amino acids one by one, this process for more than 3 amino acids of the polypeptide synthesis, the yield will be greatly reduced, and the synthesis process will produce a large number of non-target peptide impurities, the target product purity is low;

(2) In the process of peptide synthesis, each step of adding and connecting amino acids needs to be completed in an organic reagent, and the resulting organic reagent waste liquid needs to be specially treated, which not only causes great pressure on the reagent cost and waste liquid treatment cost, but also has a negative impact on environmental protection. In order to solve the above problems, the market has developed a method for preparing SNAP-8 by combining chemical and biological methods.

Acetyl hexapeptide -3 MS

according to the structural formula of SNAP-8, the protective dipeptide H-Glu-Glu-OH is first synthesized by chemical method, then the protective pentapeptide Met-Gln-Arg-Arg-Ala is synthesized by biological method, then the H-Asp(OAll)-OMe is synthesized by chemical method, then the protective dipeptide H-Glu-Glu-OH, the protective pentapeptide Met-Gln-Arg-Arg-Ala and the H-Asp(OAll)-OMe are connected in a certain way, and finally the acetyl group and amino group are added by chemical modification to obtain high purity SNAP-8.

This method can effectively reduce the steps of chemical synthesis, reduce the use of organic reagents, reduce the pollution of the environment, has the advantages of environmental protection, and provides a new idea for the synthesis of other peptides.